ZEB1 ELISA Kit (Human) : 96 Wells (OKEH02404)

Catalog No: OKEH02404 (Formerly GWB-KXLYPE)

Size:96T

Human ZEB1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ZEB1 ELISA Kit (Human) (OKEH02404) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human ZEB1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ZEB1 and incubated. The ZEB1 found in the sample or standards competes with the biotinylated ZEB1 for limited binding sites on the immobilized anti-Human ZEB1 antibody. Excess unbound biotinylated ZEB1 and sample or standard ZEB1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ZEB1 captured in the well and inversely proportional to the amount of ZEB1 which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.9% (n = 20)
  Mean Inter-assay CV%: < 9.8% (n = 20)
• ELISA Kit Component:
  • Anti-ZEB1 Microplate: 96 Wells (12 x 8 Well strips)
  • ZEB1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X MTOR-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.23 ng/mL
• Specificity: Natural and recombinant Human Zinc finger E-box-binding homeobox 1
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: ZEB1
• Gene Full Name: zinc finger E-box binding homeobox 1
• Alias Symbols: AREB6;BZP;delta-crystallin enhancer binding factor 1;DELTAEF1;FECD6;negative regulator of IL2;NIL2A;NIL-2-A zinc finger protein;posterior polymorphous corneal dystrophy 3;PPCD3;TCF8;Transcription factor 8;transcription factor 8 (represses interleukin 2 expression);ZFHEP;ZFHX1A;zinc finger E-box-binding homeobox 1;zinc finger homeodomain enhancer-binding protein.
• NCBI Gene Id: 6935
• Protein Name: P37275
• Description of Target: Zinc finger E-box-binding homeobox 1
• Uniprot ID: https://www.uniprot.org/uniprot/P37275
• Protein Accession #: NP_001121600.1
• Nucleotide Accession #: NM_001128128.2
• Protein Size (# AA): 1124
• Molecular Weight: 124 kDa