ZEB1 Antibody - middle region (OAGA02510)

Catalog No: OAGA02510

Size:100 ul

Sample (30 ug of whole cell lysate)
A: 293T
B: HeLa
5% SDS PAGE
OAGA02510 diluted at 1:1000

ZEB1 antibody Internal detects ZEB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZEB1 protein stained by ZEB1 antibody , Internal (OAGA02510) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 um.

ZEB1 antibody immunoprecipitates ZEB1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of ZEB1 protein by 2 ug of ZEB1 antibody (OAGA02510) 7.5% SDS-PAGE The immunoprecipitated ZEB1 protein was detected by ZEB1 antibody (OAGA02510) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS, 20% Glycerol (pH 7). 0.025% Proclin300 was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, ICC, IP, WB, CHIP
  Positive Controls: 293T, HeLa, HepG2, MDA-MB-231, Neuro2A, NIH-3T3, C2C12
  Cellular Localization: Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ZEB1. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Rat (89%), Chicken (89%), Bovine (94%)
• Concentration: 0.1 mg/ml
• Application Info: ICC/IF: 1:100~1000
  IP: 1:100~500
  WB: 1:500~3000
  CHIP: Assay-dependent dilution

Target Info

• Gene Symbol: ZEB1
• Gene Full Name: zinc finger E-box binding homeobox 1
• Alias Symbols: BZP, TCF8, AREB6, FECD6, NIL2A, PPCD3, ZFHEP, ZFHX1A, DELTAEF1
• NCBI Gene Id: 6935
• Description of Target: ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site.
• Uniprot ID: P37275
• Molecular Weight: 124 kDa