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Catalog No: OCOA10501
Size:20UG
Price: $179.00
SKU
OCOA10501
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ZBTB7A Protein Lysate (OCOA10501)

Datasheets/ManualsPrintable datasheet for ZBTB7A Protein Lysate (OCOA10501)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionZBTB7A HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolZBTB7A
Gene Full Namezinc finger and BTB domain containing 7A
Alias Symbolsfactor binding IST protein 1;factor that binds to inducer of short transcripts protein 1;FBI1;FBI-1;HIV-1 1st-binding protein 1;HIV-1 inducer of short transcripts binding protein;leukemia/lymphoma-related factor;LRF;lymphoma related factor;POK erythroid myeloid ontogenic factor;pokemon;pokemon 1;POZ and Krueppel erythroid myeloid ontogenic factor;TIP21;TTF-I-interacting peptide 21;ZBTB7;zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcripts binding protein;zinc finger and BTB domain-containing protein 7A;zinc finger protein 857A;ZNF857A.
NCBI Gene Id51341
Protein NameNM_015898, NP_056982, O95365
Description of TargetHomo sapiens zinc finger and BTB domain containing 7A (ZBTB7A), transcript variant 1, mRNA, Zinc finger and BTB domain-containing protein 7A, zinc finger and BTB domain-containing protein 7A [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/O95365
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_056982
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_015898
Molecular Weight61.3 kDa
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