ZBTB16 Antibody (OAGA03690)

Catalog No: OAGA03690

Size:100 ul

Sample (30 ug of whole cell lysate)
A: THP-1
7.5% SDS PAGE
diluted at 1:1000

PLZF antibody [N1N3] detects PLZF protein at nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PLZF protein stained by PLZF antibody [N1N3] diluted at 1:2000.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PLZF. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:2000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: ZBTB16
• Gene Full Name: zinc finger and BTB domain containing 16
• Alias Symbols: PLZF, ZNF145
• NCBI Gene Id: 7704
• Protein Name: zinc finger and BTB domain-containing protein 16
• Description of Target: This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.
• Uniprot ID: Q05516
• Protein Accession #: NP_001018011.1
• Nucleotide Accession #: NM_001018011.1