Human ZAP70/SYK Antibody : FITC (Phospho-Tyr493/Tyr526) (OAAQ00026)

Catalog No: OAAQ00026

Flow cytometric analysis of Jurkat cells unstained untreated cells as negative control (blue) or stained and untreated (red) or treated with pervanadate (green) using phospho-Zap70 (Tyr493)/Syk (Tyr526) antibody Zap70Y493-H11-FITC conjugate. Cat. #OAAQ00026.

Product Info

• Tested Species Reactivity: Human, Mouse
• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid. 1X PBS, 0.09% NaN3, 0.2% BSA.
• Clonality: Monoclonal
• Clone: Zap70Y493-H11
• Isotype: Rabbit IgG1k
• Host: Rabbit
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: FC
• Reconstitution and Storage: 2-8C
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr493 of human phospho Zap70
• Purification: Protein A+G
• Target Post-Translational Modification: Phospho-Tyr493/Tyr526
• Application Info: For flow cytometric staining, the suggested use of this reagent is 5 uL per million cells or 5 uL per 100 uL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
• Reference: 1. Brdicka T et al., (2005) Mol Cell Biol 25:4924-4933.
  2. Chan AC et al., (1992) Cell 71:649-662.
  3. Cheng AM et al., (1997) Proc Natl Acad Sci94:9797-9801.
  4. Deindl S et al., (2007) Cell 129:735-746.
  5. Elder ME et al., (1994) Science 264:1596-1599.
  6. Negishi I et al., (1995) Nature 376:435-438.
  7. Yokosuka T et al., (2005) Nat Immunol 6:1253-1262.

Target Info

• Gene Symbol: ZAP70, SYK
• Gene Full Name: zeta chain of T cell receptor associated protein kinase 70, spleen associated tyrosine kinase
• Alias Symbols: SRK, STD, TZK, STCD, IMD48, ADMIO2, ZAP-70
• NCBI Gene Id: 7535; 6850
• Protein Name: tyrosine-protein kinase ZAP-70, tyrosine-protein kinase SYK
• Description of Target: This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
  This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P43403, P43405
• Protein Accession #: NP_001070.2
• Nucleotide Accession #: NM_001079.3