ZAP70 Antibody (OAAB18686)

Catalog No: OAAB18686

Size:400ul

All lanes: Anti-ZAP70 Antibody at 1: 4000 dilution
Lane 1: Jurkat whole cell lysates
Lane 2: MOLT-4 whole cell lysates
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 70 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Monoclonal
• Clone: 1475CT217.34.65
• Isotype: IgG1, k
• Host: Mouse
• Application: WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ZAP70 antibody is generated from a mouse immunized with a recombinant protein.
• Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Application Info: IF: 1:25
  FC: 1:25
  WB: 1:4000
• Reference: Chan A.C.,et al.Cell 71:649-662(1992).
  Kuroyama H.,et al.Biochem. Biophys. Res. Commun. 315:935-941(2004).
  Hillier L.W.,et al.Nature 434:724-731(2005).
  Arpaia E.,et al.Cell 76:947-958(1994).
  Isakov N.,et al.J. Exp. Med. 181:375-380(1995).
  

Target Info

• Gene Symbol: ZAP70
• Alias Symbols: SRK, STD, TZK, STCD, IMD48, ADMIO2, ZAP-70
• NCBI Gene Id: 7535
• Description of Target: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).
• Uniprot ID: P43403
• Molecular Weight: 70 kDa