ZAK ELISA Kit (Human) (OKEH06551)

Catalog No: OKEH06551

Size:96T

Human ZAK ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology ZAK ELISA Kit (Human) (OKEH06551) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-ZAK antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ZAK and incubated. The ZAK found in the sample or standards competes with the biotinylated ZAK for limited binding sites on the immobilized anti-ZAK antibody. Excess unbound biotinylated ZAK and sample or standard ZAK is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ZAK captured in the well and inversely proportional to the amount of ZAK which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 111%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <= 6.9% (n = 20)
  Mean Inter-Assay Precision - <= 9.9% (n = 20)
• ELISA Kit Component:
  • H3F3C Microplate: 96 Wells (12 x 8 Well strips)
  • H3F3C Lyophilized Standard: 2
  • 100X Biotinylated H3F3C Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.156 ng/mL
• Specificity: Natural and recombinant Human Mitogen-activated protein kinase kinase kinase MLT
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: MAP3K20
• Gene Full Name: mitogen-activated protein kinase kinase kinase 20
• Alias Symbols: AZK;CNM6;HCCS-4;human cervical cancer suppressor gene 4 protein;Leucine zipper- and sterile alpha motif-containing kinase;mitogen-activated protein kinase kinase kinase 20;mitogen-activated protein kinase kinase kinase MLT;mixed lineage kinase 7;mixed lineage kinase with a leucine zipper and a sterile alpha motif;Mixed lineage kinase-related kinase;mixed lineage kinase-related kinase MRK-beta;MLK7;mlklak;MLK-like mitogen-activated protein triple kinase;MLT;MLTK;MLTKalpha;MLTKbeta;MRK;pk;SFMMP;sterile alpha motif and leucine zipper containing kinase AZK;Sterile alpha motif- and leucine zipper-containing kinase AZK;ZAK;ZAK1 homolog, leucine zipper and sterile-alpha motif kinase.
• NCBI Gene Id: 51776
• Protein Name: Q9NYL2
• Description of Target: Mitogen-activated protein kinase kinase kinase 20
• Uniprot ID: https://www.uniprot.org/uniprot/Q9NYL2
• Protein Accession #: NP_001159764
• Nucleotide Accession #: NM_001166292