YY1 Antibody (OAAB17748)

Catalog No: OAAB17748

Size:400ul

YY1 Antibody (Cat. #OAAB17748) western blot analysis in Daudi and U251 cell line lysates (35ug/lane).
This demonstrates the YY1 antibody detected the YY1 protein (arrow).
Dilution: 1:1000

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Monoclonal
• Isotype: IgG2a
• Host: Mouse
• Application: WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: Purified His-tagged YY1 protein was used to produced this monoclonal antibody.
• Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Application Info: IHC-P: 1:25
  WB: 1:500~1:1000
  WB: 1:1000
• Reference: Dephoure N., et al. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
  Kim J., et al. Genomics 93:152-158(2009).
  Burkard T.R., et al. BMC Syst. Biol. 5:17-17(2011).
  Chen L., et al. J. Biol. Chem. 286:11283-11289(2011).
  Mayya V., et al. Sci. Signal. 2:RA46-RA46(2009).

Target Info

• Gene Symbol: YY1
• Alias Symbols: DELTA, NF-E1, UCRBP, GADEVS, INO80S, YIN-YANG-1
• NCBI Gene Id: 7528
• Description of Target: Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions).
  Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements.
• Uniprot ID: P25490
• Protein Accession #: NP_003394.1
• Molecular Weight: 45 kDa