YWHAZ Polyclonal Antibody (Human) (ATP00015_T100)

Catalog No: ATP00015_T100

Size:100ul

20 ug of the indicated human whole cell extract was loaded onto a 4-20% SDS-PAGE gel. After electrophoresis, proteins were transferred to a PVDF membrane and probed with 3 ug/mL of antibody. Secondary detection was performed with 0.08 ug/mL of HRP-conjugated donkey anti-rabbit IgG antibody. Images were captured after development of membrane following exposure to AvivaBlot™ Femto ECL Reagent (OKCF00002).

Immunogen: Recombinant protein representing aa 2-244 of human  YWHAZ (green) depicted within in the full length structure prediction via AlphaFold. (https://alphafold.ebi.ac.uk/).

Product Info

• Predicted Species Reactivity: Human
• Product Format: PBS w/ 0.09% sodium azide
• Clonality: Polyclonal
• Isotype: IgG
• Application: WB;ICC
• Reconstitution and Storage: Store at -20C
• Immunogen: Recombinant Human YWHAZ aa 2-244
• Purification: Protein A
• Concentration: 1 mg/ml

Target Info

• Gene Symbol: 14-3-3 protein zeta/delta
• Gene Full Name: YWHAZ
• NCBI Gene Id: 7534
• Description of Target: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:14578935, PubMed:15071501, PubMed:15644438, PubMed:16376338, PubMed:16959763, PubMed:31024343, PubMed:9360956). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:35662396). Binding generally results in the modulation of the activity of the binding partner (PubMed:35662396). Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB (PubMed:35662396). Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation (PubMed:16959763). In neurons, regulates spine maturation through the modulation of ARHGEF7 activity (By similarity).
• Uniprot ID: P63104
• Protein Accession #: P63104
• Molecular Weight: 28 kDa (245 aa)