YWHAZ ELISA Kit (Human) (OKCA02073)

Catalog No: OKCA02073

Size:96 Wells

Human YWHAZ ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	93	89-99
  Serum (n=4)	1:2	89	85-95
  Serum (n=4)	1:4	97	90-105
  Serum (n=4)	1:8	95	87-100

• ELISA Kit Principle: Aviva Systems Biology YWHAZ ELISA Kit (Human) (OKCA02073) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for YWHAZ has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for YWHAZ is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample YWHAZ captured in well.
• ELISA Kit Range: 0.625 ng/mL-40 ng/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	97	92-103
  EDTA plasma (n=4)	92	88-95

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-YWHAZ Microplate: 96 Wells (12 x 8 Well strips)
  • YWHAZ Lyophilized Standard: 2 x 40 ng
  • 100X Biotinylated YWHAZ Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.156 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: YWHAZ
• Alias Symbols: HEL4, YWHAD, KCIP-1, HEL-S-3, POPCHAS, HEL-S-93, 14-3-3-zeta
• NCBI Gene Id: 7534
• Protein Name: 14-3-3 protein zeta/delta
• Description of Target: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
• Uniprot ID: P63104
• Protein Accession #: NP_001129171
• Nucleotide Accession #: NM_001135699