YWHAG Antibody (OAAF02792)

Catalog No: OAAF02792 (Formerly GWB-ASD984)

Size:100 ug

Western blot analysis of extracts from K562 cells, treated with insulin 0.01U/ml 15', using 14-3-3 gamma Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using 14-3-3 gamma Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of COS7 cells, using 14-3-3 gamma Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human 14-3-3 gamma.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: 14-3-3 γ antibody detects endogenous levels of total 14-3-3 γ protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:1000
• Reference: Autieri M.V., DNA Cell Biol. 18:555-564(1999).
  Horie M., Genomics 60:241-243(1999).
  Hillier L.W., Nature 424:157-164(2003).

Target Info

• Gene Symbol: YWHAG
• Gene Full Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma
• Alias Symbols: 14-3-3 protein gamma;14-3-3GAMMA;DEE56;EIEE56;KCIP-1;PPP1R170;protein kinase C inhibitor protein 1;protein phosphatase 1, regulatory subunit 170;tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide.
• NCBI Gene Id: 7532
• Protein Name: 14-3-3 protein gamma
• Description of Target: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
• Uniprot ID: P61981
• Protein Accession #: NP_036611.2
• Molecular Weight: 28 kDa