YWHAE Polyclonal Antibody (Human) (ATP00014_T100)

Catalog No: ATP00014_T100

Size:100ul

20 ug of the indicated human whole cell extract was loaded onto a 4-20% SDS-PAGE gel. After electrophoresis, proteins were transferred to a PVDF membrane and probed with 3 ug/mL of antibody. Secondary detection was performed with 0.08 ug/mL of HRP-conjugated donkey anti-rabbit IgG antibody. Images were captured after development of membrane following exposure to AvivaBlot™ Femto ECL Reagent (OKCF00002).

Immunogen: Recombinant protein representing aa 2-255 of human  YWHAE (green) depicted within in the full length structure prediction via AlphaFold. (https://alphafold.ebi.ac.uk/).

Product Info

• Predicted Species Reactivity: Human
• Product Format: PBS w/ 0.09% sodium azide
• Clonality: Polyclonal
• Isotype: IgG
• Application: WB;ICC
• Reconstitution and Storage: Store at -20C
• Immunogen: Recombinant YWHAE aa 2-255
• Purification: Protein A
• Concentration: 1 mg/ml

Target Info

• Gene Symbol: 14-3-3 protein epsilon
• Gene Full Name: YWHAE
• NCBI Gene Id: 7531
• Description of Target: Promotes the exchange of Ras-bound GDP by GTP (PubMed:8493579). Probably by promoting Ras activation, regulates phosphorylation of MAP kinase MAPK3 in response to EGF (PubMed:17339331). Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity (By similarity).
• Uniprot ID: Q07889
• Molecular Weight: 29 kDa (255 aa)