YWHAE ELISA Kit (Human) (OKCA01073)

Catalog No: OKCA01073

Size:96 Wells

Human YWHAE ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	86	81-92
  1:2	98	92-103
  1:4	94	88-98
  1:8	93	87-96

• ELISA Kit Principle: Aviva Systems Biology YWHAE ELISA Kit (Human) (OKCA01073) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for YWHAE has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for YWHAE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample YWHAE captured in the well.
• ELISA Kit Range: 78 - 5,000 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	87	85-93
  EDTA Plasma (n=4)	103	98-106

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01073 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01073 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • YWHAE Microplate: 96 Wells (12 x 8 Well strips)
  • YWHAE Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated YWHAE Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 19.5 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: YWHAE
• Gene Full Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon
• Alias Symbols: MDS, HEL2, MDCR, KCIP-1, 14-3-3E
• NCBI Gene Id: 7531
• Protein Name: 14-3-3 protein epsilon
• Description of Target: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
• Uniprot ID: P62258
• Protein Accession #: NP_006752.1
• Nucleotide Accession #: NM_006761.4