YTHDF2 Antibody - C-terminal region (ARP67917_P050)

Catalog No: ARP67917_P050

Host: Rabbit
Target Name: YTHDF2
Sample Type: Jurkat Whole Cell lysates
Antibody Dilution: 1.0ug/mlYTHDF2 is supported by BioGPS gene expression data to be expressed in Jurkat

Host: Rabbit
Target Name: YTHDF2
Sample Tissue: Human Hela Whole Cell
Antibody Dilution: 1ug/ml

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human YTHDF2
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: QEVPLEKAKQVLKIIASYKHTTSIFDDFSHYEKRQEEEESVKKERQGRGK
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-YTHDF2 (ARP67917_P050) antibody is Catalog # AAP67917
• Sample Type Confirmation:

  YTHDF2 is supported by BioGPS gene expression data to be expressed in Jurkat

• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Publications:

  A potentially abundant junctional RNA motif stabilized by m6A and Mg2. Nat Commun. 9, 2761 (2018). 30018356

  Context-dependent functional compensation between Ythdf m6A reader proteins. Genes Dev. 34, 1373-1391 (2020) 32943573

  Control of Early B Cell Development by the RNA N6-Methyladenosine Methylation. Cell Rep. 31, 107819 (2020). 32610122

  EGFR/SRC/ERK-stabilized YTHDF2 promotes cholesterol dysregulation and invasive growth of glioblastoma. Nat Commun. 12, 177 (2021). 33420027

  m6A enhances the phase separation potential of mRNA. Nature. 571, 424-428 (2019). 31292544

  m6A mRNA methylation regulates AKT activity to promote the proliferation and tumorigenicity of endometrial cancer. Nat Cell Biol. 20, 1074-1083 (2018). 30154548

  YTHDF2/3 Are Required for Somatic Reprogramming through Different RNA Deadenylation Pathways. Cell Rep. 32, 108120 (2020). 32905781

Target Info

• Gene Symbol: YTHDF2
• Alias Symbols: DF2, CAHL, HGRG8, NY-REN-2
• NCBI Gene Id: 51441
• Protein Name: YTH domain family protein 2
• Description of Target: This gene encodes a member of the YTH (YT521-B homology) superfamily containing YTH domain. The YTH domain is typical for the eukaryotes and is particularly abundant in plants. The YTH domain is usually located in the middle of the protein sequence and may function in binding to RNA. In addition to a YTH domain, this protein has a proline rich region which may be involved in signal transduction. An Alu-rich domain has been identified in one of the introns of this gene, which is thought to be associated with human longevity. In addition, reciprocal translocations between this gene and the Runx1 (AML1) gene on chromosme 21 has been observed in patients with acute myeloid leukemia. This gene was initially mapped to chromosome 14, which was later turned out to be a pseudogene.
• Uniprot ID: Q9Y5A9
• Protein Accession #: NP_057342
• Nucleotide Accession #: NM_016258
• Protein Size (# AA): 579
• Molecular Weight: 62 kDa
• Protein Interactions: UBC; RPA3; RPA2; RPA1; RNF2; BMI1; EIF3H; EIF3A; FBXO6; TARDBP; HIPK4; VCAM1; FN1; EPAS1; ESR1; APP; SMAD3; CAND1; CUL3; ELAVL1; POT1; MEPCE; HNRNPH1; HNRNPA1;