YOD1 Antibody - C-terminal region (OAAB07550)

Catalog No: OAAB07550

Size:400ul

YOD1 Antibody (C-term) (Cat. #OAAB07550) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the YOD1 antibody detected the YOD1 protein (arrow).

YOD1 Antibody (C-term) (Cat. #OAAB07550)immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of YOD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of YOD1 Antibody (C-term)(Cat#OAAB07550) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human, Zebrafish
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, IF
• Additional Information: Precautions: YOD1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Able to cleave both polyubiquitin and di- ubiquitin
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This YOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-347 amino acids from the C-terminal region of human YOD1.
• Predicted Homology Based on Immunogen Sequence: Human; Zebrafish
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  YOD1 is strongly supported by BioGPS gene expression data to be expressed in K562

• Application Info: WB: 1:2000
  WB: 1:1000
  IHC-P: 1:50~100
  IF: 1:10~50

Target Info

• Gene Symbol: YOD1
• Alias Symbols: DUBA8, OTUD2, PRO0907
• NCBI Gene Id: 55432
• Protein Name: Ubiquitin thioesterase OTU1
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q5VVQ6
• Protein Accession #: NP_001263249.1
• Molecular Weight: 38322 Da