Catalog No: OKEH08279
Size:96T
Price: $725.00
SKU
OKEH08279
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology YBX3 ELISA Kit (Human) (OKEH08279) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for YBX3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for YBX3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample YBX3 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.78-50ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=3.1% (n = 20) Mean Inter-assay CV%: <=10.8% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: Binds to the GM-CSF promoter. Seems to act as a repressor. Binds also to full length mRNA and to short RNA sequences containing the consensus site 5'-UCCAUCA-3'. May have a role in translation repression. | ||||||||||||||||||||||
:: | Subcellular Location: Cytoplasm Nucleus | ||||||||||||||||||||||
:: | Unigene: Hs.221889 SMR: P16989 String: 9606.ENSP00000228251 | ||||||||||||||||||||||
:: | KEGG: hsa:8531 MIM: 603437 Pfam: PF00313 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 0.45ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Y-box-binding protein 3 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | YBX3 |
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Gene Full Name | Y-box binding protein 3 |
Alias Symbols | cold shock domain-containing protein A;cold-shock domain containing A1;cold-shock domain protein A;CSDA;CSDA1;DBPA;DNA-binding protein A;single-strand DNA-binding protein NF-GMB;Y-box-binding protein 3;ZO-1-associated nucleic acid-binding protein;ZONAB. |
NCBI Gene Id | 8531 |
Protein Name | P16989 |
Description of Target | Y-box-binding protein 3 |
Uniprot ID | https://www.uniprot.org/uniprot/P16989 |
Protein Accession # | NP_001138898.1 |
Nucleotide Accession # | NM_001145426.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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