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Catalog No: OKEH08279
Size:96T
Price: $725.00
SKU
OKEH08279
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology YBX3 ELISA Kit (Human) (OKEH08279) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for YBX3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for YBX3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample YBX3 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=3.1% (n = 20)
Mean Inter-assay CV%: <=10.8% (n = 20)
ELISA Kit Component
ComponentAmount
YBX3 Microplate96 Wells (12 x 8 Well strips)
YBX3 Lyophilized Standard2
100X Biotinylated YBX3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Binds to the GM-CSF promoter. Seems to act as a repressor. Binds also to full length mRNA and to short RNA sequences containing the consensus site 5'-UCCAUCA-3'. May have a role in translation repression.
::Subcellular Location: Cytoplasm Nucleus
::Unigene: Hs.221889
SMR: P16989
String: 9606.ENSP00000228251
::KEGG: hsa:8531
MIM: 603437
Pfam: PF00313
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.45ng/mL
SpecificityNatural and recombinant Human Y-box-binding protein 3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolYBX3
Gene Full NameY-box binding protein 3
Alias Symbolscold shock domain-containing protein A;cold-shock domain containing A1;cold-shock domain protein A;CSDA;CSDA1;DBPA;DNA-binding protein A;single-strand DNA-binding protein NF-GMB;Y-box-binding protein 3;ZO-1-associated nucleic acid-binding protein;ZONAB.
NCBI Gene Id8531
Protein NameP16989
Description of TargetY-box-binding protein 3
Uniprot IDhttps://www.uniprot.org/uniprot/P16989
Protein Accession #NP_001138898.1
Nucleotide Accession #NM_001145426.1
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