YBX2 Antibody - middle region (OAGA05311)

Catalog No: OAGA05311

Size:100 ul

Sample (30 ug of whole cell lysate)
A: IMR32
10% SDS PAGE
diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: mouse testis
10% SDS PAGE
diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using YBX2 antibody at 1:250 dilution.

YBX2 antibody [N2C2], Internal detects YBX2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: YBX2 protein stained by YBX2 antibody [N2C2], Internal diluted at 1:500.
Blue: Hoechst 33343 staining.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human YBX2. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.43 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: YBX2
• Gene Full Name: Y-box binding protein 2
• Alias Symbols: DBPC, MSY2, CSDA3, CONTRIN
• NCBI Gene Id: 51087
• Protein Name: Y-box-binding protein 2
• Description of Target: This gene encodes a nucleic acid binding protein which is highly expressed in germ cells. The encoded protein binds to a Y-box element in the promoters of certain genes but also binds to mRNA transcribed from these genes. Pseudogenes for this gene are located on chromosome 10 and 15.
• Uniprot ID: Q9Y2T7
• Protein Accession #: NP_057066.2
• Nucleotide Accession #: NM_015982.3