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Catalog No: OKCD04500
Size:96WELLS
Price: $675.00
SKU
OKCD04500
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Datasheets/ManualsPrintable datasheet for y+LAT1 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to L-Type Amino Acid Transporter 1 (y+LAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to L-Type Amino Acid Transporter 1 (y+LAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain L-Type Amino Acid Transporter 1 (y+LAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of L-Type Amino Acid Transporter 1 (y+LAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level L-Type Amino Acid Transporter 1 (y+LAT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level L-Type Amino Acid Transporter 1 (y+LAT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
NTCP Microplate96 Wells (12 x 8 Well strips)
NTCP Lyophilized Standard2
100X NTCP HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity0.114 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolY+LAT1
Alias SymbolsLPI, LAT3, MOP-2, Y+LAT1, y+LAT-1
NCBI Gene Id9056
Protein NameY+L amino acid transporter 1
Description of TargetThe protein encoded by this gene is the light subunit of a cationic amino acid transporter. This sodium-independent transporter is formed when the light subunit encoded by this gene dimerizes with the heavy subunit transporter protein SLC3A2. This transporter is found in epithelial cell membranes where it transfers cationic and large neutral amino acids from the cell to the extracellular space. Defects in this gene are a cause of lysinuric protein intolerance (LPI). Alternative splicing results in multiple transcript variants.
Uniprot IDQ9UM01
Protein Accession #NP_001119577.1
Nucleotide Accession #NM_001126105.2
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