XRCC5 Antibody (OAGA08800)

Catalog No: OAGA08800

Size:100 ul

Detection of human Ku80 protein in HeLa whole cell lysate using Ku80 149.8 monoclonal antibody.

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: 293T
D: A431
E: HeLa
F: HepG2
G: H1299
H: HCT116
I: MCF-7
J: NT2D1
K: PC-3
L: U87-MG
7.5% SDS PAGE
diluted at 1:10000

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
7.5% SDS PAGE
diluted at 1:10000

Ku80 antibody [149.8] detects Ku80 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ku80 protein stained by Ku80 antibody [149.8] diluted at 1:500.
Blue: Hoechst 33343 staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Monoclonal
• Clone: 149.8
• Isotype: IgG
• Host: Mouse
• Application: ICC, IF, WB
• Reconstitution and Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.
• Immunogen: Recombinant antigen containing the carboxy terminal portion of human Ku80 protein
• Purification: Protein G purified
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:1000-1:10000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: PBS

Target Info

• Gene Symbol: XRCC5
• Gene Full Name: X-ray repair cross complementing 5
• Alias Symbols: KU80, KUB2, Ku86, NFIV, KARP1, KARP-1
• NCBI Gene Id: 7520
• Protein Name: X-ray repair cross-complementing protein 5
• Description of Target: The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
• Uniprot ID: P13010
• Protein Accession #: NP_066964.1
• Nucleotide Accession #: NM_021141.3