Catalog No: OKCD01898 (Formerly GWB-KBBFX4)
Size:96WELLS
Price: $675.00
SKU
OKCD01898
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Datasheets/ManualsPrintable datasheet for XRCC2 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)90-103%88-95%92-102%83-97%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to X-Ray Repair Cross Complementing 2 (XRCC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to X-Ray Repair Cross Complementing 2 (XRCC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain X-Ray Repair Cross Complementing 2 (XRCC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of X-Ray Repair Cross Complementing 2 (XRCC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum80-10295
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level X-Ray Repair Cross Complementing 2 (XRCC2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level X-Ray Repair Cross Complementing 2 (XRCC2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-XRCC2 Microplate96 Wells (12 x 8 Well Strips)
XRCC2 Lyophilized Standard2 x 20 ng
100X Biotinylated XRCC2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.114 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of X-Ray Repair Cross Complementing 2 (XRCC2). No significant cross-reactivity or interference between X-Ray Repair Cross Complementing 2 (XRCC2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolXRCC2
Gene Full NameX-ray repair cross complementing 2
Alias SymbolsFANCU, POF17, SPGF50
NCBI Gene Id7516
Protein NameDNA repair protein XRCC2
Description of TargetInvolved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.
Uniprot IDO43543
Protein Accession #NP_005422.1
Nucleotide Accession #NM_005431.1.
Protein Size (# AA)280
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