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Catalog No: OKCD04058
Size:96WELLS
Price: $800.00
SKU
OKCD04058
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XPC Chemi-Luminescent ELISA Kit (Human) (OKCD04058)

Datasheets/ManualsPrintable datasheet for XPC Chemi-Luminescent ELISA Kit (Human) (OKCD04058)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Xeroderma Pigmentosum, Complementation Group C (XPC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Xeroderma Pigmentosum, Complementation Group C (XPC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Xeroderma Pigmentosum, Complementation Group C (XPC) level in the sample or standard.
ELISA Kit Range13.7-10,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Xeroderma Pigmentosum, Complementation Group C (XPC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Xeroderma Pigmentosum, Complementation Group C (XPC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
XPC Microplate96 Wells (12 x 8 Well strips)
XPC Lyophilized Standard2
100X XPC Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity0.156 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolXPC
Gene Full NameXPC complex subunit, DNA damage recognition and repair factor
Alias SymbolsDNA repair protein complementing XP-C cells;mutant xeroderma pigmentosum group C;p125;RAD4;Xeroderma pigmentosum group C-complementing protein;xeroderma pigmentosum, complementation group C;XP3;XPCC.
NCBI Gene Id7508
Protein NameDNA repair protein complementing XP-C cells
Description of TargetInvolved in global genome nucleotide excision repair (GG-NER) by acting as damage sensing and DNA-binding factor component of the XPC complex. Has only a low DNA repair activity by itself which is stimulated by RAD23B and RAD23A. Has a preference to bind DNA containing a short single-stranded segment but not to damaged oligonucleotides. This feature is proposed to be related to a dynamic sensor function: XPC can rapidly screen duplex DNA for non-hydrogen-bonded bases by forming a transient nucleoprotein intermediate complex which matures into a stable recognition complex through an intrinsic single-stranded DNA-binding activity.
Uniprot IDQ01831
Protein Accession #NP_004619
Nucleotide Accession #NM_004628
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