Size:96 Wells
$469.00
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SKU
OKEH03211
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XDH ELISA Kit (Rat) (OKEH03211)

Catalog#: OKEH03211
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Rat
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology XDH ELISA Kit (Rat) (OKEH03211) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Xdh has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Xdh is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Xdh captured in well.
Kit Range 0.156 - 10ng/mL
Kit Component
ComponentAmount
Anti-Xdh Microplate96 Wells (12 x 8 Well strips)
Xdh Lyophilized Standard2
100X Biotinylated Xdh Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity 0.078 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Rat Xanthine dehydrogenase/oxidase
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol XDH
Official Gene Full Name xanthine dehydrogenase
Alias Symbols Xanthine dehydrogenase/oxidase
NCBI Gene Id 497811
Protein Name Xanthine dehydrogenase/oxidase
Description of Target Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species.
Swissprot Id P22985
Protein Accession # NP_058850.1
Nucleotide Accession # NM_017154.1
Protein Size (# AA) 1331
Molecular Weight 146 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express Xdh.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express Xdh.
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