Catalog No: OCOA01433
Size:20UG
Price: $179.00
SKU
OCOA01433
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XBP1 Protein Lysate (OCOA01433)

Datasheets/ManualsPrintable datasheet for XBP1 Protein Lysate (OCOA01433)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionXBP1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolXBP1
Gene Full NameX-box binding protein 1
Alias Symbolstax-responsive element-binding protein 5;TREB5;TREB-5;X-box-binding protein 1;XBP-1;XBP2.
NCBI Gene Id7494
Protein NameA0A024R1F0, NM_005080, NP_005071, P17861
Description of TargetHomo sapiens X-box binding protein 1 (XBP1), transcript variant 1, mRNA, X-box binding protein 1, isoform CRA_b, X-box-binding protein 1, X-box-binding protein 1 isoform XBP1(U) [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P17861
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_005071
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_005080
Molecular Weight28.7 kDa
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