XBP1 Antibody (OAPB00381)

Catalog No: OAPB00381

Size:100UG

Western blot analysis of XBP-1 in HepG2 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Liquid. XBP-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: XBP-1 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Anti-XBP-1 antibody (3687) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human XBP-1. The immunogen is located within amino acids 40 - 90 of XBP-1.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-XBP1 (OAPB00381) antibody is Catalog # OEPB02500
• Application Info: XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.;Calfon M, Zeng H, Urano F, et al. IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA. Nature 2002; 415:92-6.;Haze K, Yoshida H, Yanagi H, et al. Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic stress. Mol. Cell. Biol. 1999; 10:3787-99.;Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.

Target Info

• Gene Symbol: XBP1
• Gene Full Name: X-box binding protein 1
• Alias Symbols: tax-responsive element-binding protein 5;TREB5;TREB-5;X-box-binding protein 1;XBP-1;XBP2.
• NCBI Gene Id: 7494
• Protein Name: X-box-binding protein 1
• Description of Target: Functions as a transcription factor during endoplasmic reticulum (ER) stress by regulating the unfolded protein response (UPR). Required for cardiac myogenesis and hepatogenesis during embryonic development, and the development of secretory tissues such as exocrine pancreas and salivary gland (By similarity). Involved in terminal differentiation of B lymphocytes to plasma cells and production of immunoglobulins (PubMed:11460154). Modulates the cellular response to ER stress in a PIK3R-dependent manner (PubMed:20348923). Binds to the cis-acting X box present in the promoter regions of major histocompatibility complex class II genes (PubMed:8349596). Involved in VEGF-induced endothelial cell (EC) proliferation and retinal blood vessel formation during embryonic development but also for angiogenesis in adult tissues under ischemic conditions. Functions also as a major regulator of the UPR in obesity-induced insulin resistance and type 2 diabetes for the management of obesity and diabetes prevention (By similarity).
• Uniprot ID: P17861
• Protein Accession #: NP_001073007.1; NP_005071.2
• Nucleotide Accession #: NM_001079539.1; NM_005080.3
• Molecular Weight: Predicted: 28/40kD Observed: 28/40 kD