WT1 Recombinant Protein (OPCD00233)

Catalog No: OPCD00233

WT1 Recombinant Protein (OPCD00233) using SDS-PAGE

WT1 Recombinant Protein (OPCD00233) using SDS-PAGE

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Application: Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
  Residues: Leu310-Leu449
  Isoelectric Point: 10.3
  Subcellular Location: Nucleus, Cytoplasm
• Reconstitution and Storage: 2°C to 8°C|-80°C
• Concentration: 200 ug/mL (prior to lyoph)
• Purity: > 90%
• Source: E.coli
• Tag: N-terminal His and GST Tag

Target Info

• Gene Symbol: WT1
• Gene Full Name: WT1 transcription factor
• Alias Symbols: AWT1;GUD;NPHS4;WAGR;Wilms tumor 1;Wilms tumor protein;WIT-2;WT33.
• NCBI Gene Id: 7490
• Protein Name: Wilms tumor protein
• Description of Target: Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).
• Uniprot ID: P19544
• Protein Accession #: NP_000369.3
• Nucleotide Accession #: NM_000378.4
• Protein Size (# AA): Leu310~Leu446
• Molecular Weight: 47kDa