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WT1 ELISA Kit (Mouse) (OKEH03610)

96 Wells
$609.00
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Gene Symbol:
WT1
Official Gene Full Name:
Wilms tumor 1 homolog
NCBI Gene Id:
22431
Alias Symbols:
D630046I19Rik, Wilms tumor protein homolog, Wt-1
Description of Target:
Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.3 Publications
Protein Name:
Wilms tumor protein homolog
Swissprot Id:
P22561
Predicted Species Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity:
0.039 ng/mL
Kit Range:
0.078 - 5 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: <= 4.6% (n = 20)
Mean Inter-assay CV%: <= 6.9% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology WT1 ELISA Kit (Mouse) (OKEH03610) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Wt1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Wt1 and incubated. The Wt1 found in the sample or standards competes with the biotinylated Wt1 for limited binding sites on the immobilized anti-human Wt1 antibody. Excess unbound biotinylated Wt1 and sample or standard Wt1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Wt1 captured in the well and inversely proportional to the amount of Wt1 which was contained in the sample or standard.
Kit Component:
ComponentAmount
Anti-Wt1 Microplate96 Wells (12 x 8 Well strips)
Wt1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Wt1-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Wt1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Wt1.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural Mouse Wilms tumor protein homolog. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Competitive Immunoassay
Molecular Weight:
35 kDa
Protein Size (# AA):
305

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