WT1 Antibody (OACA04248)

Catalog No: OACA04248

Immunohistochemistry of paraffin-embedded human endometrial cancer at dilution 1:100

Immunohistochemistry of paraffin-embedded human placenta tissue at dilution 1:100

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC
• Reconstitution and Storage: Shipped at 4C. Upon delivery aliquot and store at -20C or -80C. Avoid repeated freeze.
• Immunogen: Recombinant human Wilms tumor protein (1-449AA)
• Purification: Caprylic Acid Ammonium Sulfate Precipitation purified
• Preservative: 0.03% Proclin 300
• Formulation: 50% Glycerol, 0.01M PBS, PH 7.4
• Reference: [1]"The genomic organization and expression of the WT1 gene." Gessler M., Konig A., Bruns G.A.P. Genomics 12:807-813(1992). [2]"Alternative splicing and genomic structure of the Wilms tumor gene WT1." Haber D.A., Sohn R.L., Buckler A.J., Pelletier J., Call K.M., Housman D.E. Proc. Natl. Acad. Sci. U.S.A. 88:9618-9622(1991). [3]"Homozygous deletion in Wilms tumours of a zinc-finger gene identified by chromosome jumping." Gessler M., Poustka A., Cavenee W., Neve R.L., Orkin S.H., Bruns G.A.P. Nature 343:774-778(1990).

Target Info

• Gene Symbol: WT1
• Gene Full Name: Wilms tumor 1
• Alias Symbols: GUD, AWT1, WAGR, WT33, NPHS4, WIT-2
• NCBI Gene Id: 7490
• Protein Name: Wilms tumor protein
• Description of Target: Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
• Uniprot ID: P19544
• Protein Accession #: NP_000369
• Nucleotide Accession #: NM_000378