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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for WT1 Antibody (OAAF08361) |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human |
Product Format | PBS, pH7.2, 0.03% Proclin 300, 50% glycerol |
Clonality | Monoclonal |
Clone | ABT-WT1 |
Isotype | IgG |
Host | Mouse |
Application | Immunohistochemistry |
Additional Information | Retrieval buffer: TRIS-EDTA of pH8.0 |
:: | Description: Mouse monoclonal to Wilms' Tumor 1 (WT1) |
Reconstitution and Storage | -20°C |
Immunogen | Synthetic peptide |
Purification | Immunogen affinity purified |
Specificity | Anti-Wilms' Tumor 1 (WT1) [ABT-WT1] detects endogenous levels of total Wilms' Tumor 1 (WT1) protein. |
Application Info | IHC 1:200 - 1:400 |
Gene Symbol | WT1 |
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Gene Full Name | WT1 transcription factor |
Alias Symbols | AWT1;GUD;NPHS4;WAGR;Wilms tumor 1;Wilms tumor protein;WIT-2;WT33. |
NCBI Gene Id | 7490 |
Protein Name | Wilms tumor protein |
Description of Target | Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921). |
Uniprot ID | P19544 |
Protein Accession # | NP_000369.3 |
Nucleotide Accession # | NM_001198551.1 |
Molecular Weight | 55 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "WT1 Antibody (OAAF08361)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".
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How long will it take to receive "WT1 Antibody (OAAF08361)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "WT1 Antibody (OAAF08361)" provided in?
This item is provided in "PBS, pH7.2, 0.03% Proclin 300, 50% glycerol".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "WT1 Antibody (OAAF08361)"?
This target may also be called "AWT1;GUD;NPHS4;WAGR;Wilms tumor 1;Wilms tumor protein;WIT-2;WT33." in publications.
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What is the shipping cost for "WT1 Antibody (OAAF08361)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "WT1 Antibody (OAAF08361)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "WT1 Antibody (OAAF08361)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "55 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "WT1 Antibody (OAAF08361)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "WT1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "WT1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "WT1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "WT1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "WT1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "WT1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.