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WT1 Antibody - middle region (Phospho-E361) (OAAB08206)

400ul
$390.00
In Stock
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Gene Symbol:
WT1
NCBI Gene Id:
7490
Protein Name:
Wilms tumor protein
Swissprot Id:
P19544
Protein Accession #:
NP_000369.3
Alias Symbols:
WT1, Wilms tumor protein, WT33
Replacement Item:
This antibody may replace item sc-15421 from Santa Cruz Biotechnology.
Description of Target:
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
Molecular Weight:
49 kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Application:
WB, IHC-P, IF, FC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express WT1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express WT1.
Immunogen:
This WT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the Central region of human WT1.
Predicted Species Reactivity:
Human, Mouse
Predicted Homology Based on Immunogen Sequence:
Xenopus, Pig, Rat
Product Format:
Liquid
Reconstitution and Storage:
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Approximately 0.5mg/ml. Actual concentration varies with each lot.
Datasheets/Manuals:
Printable datasheet for OAAB08206
Isotype:
Ig
Application Info:
WB: 1:1000
FC: 1:25
IHC-P: 1:50~100
IF: 1:10~50
:::
Cellular Location: Nucleus. Nucleus, nucleolus. Cytoplasm. Note=Shuttles between nucleus and cytoplasm. Isoform 4: Nucleus, nucleoplasm.
Tissue Location: Expressed in the kidney and a subset of hematopoietic cells.

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