- Kit Component:
- WST-1（powder） 1 tube Electron coupling reagent 1ml
- Kit Detection Method:
- Colorimetric, OD450 nm
- Printable datasheet for OKBB00004
- Reconstitution and Storage:
- At -20C in dark for one year. After reconstituting, WST-1 solution can be stored at 4C in dark for one week, at -20C in dark for half a year（avoid repeatedly freezing and thawing）.
- Notes: 1.If cell culture time is too long, please pay attention to the evaporation issue. There are two solutions for this. a. Avoid using the outmost wells of the plate, and add PBS buffer, water or culture fluid instead; b. or place 96 wells near by the water in incubator. 2.Please wear transparent gloves when operate.
- 1. WST-1 Cell Proliferation and Cytotoxicity Assay Kit is a sensitive and accurate assay for cell cytotoxicity and proliferation. The kit components are sufficient for performing up to 100 assays.
2. WST-1 assay is much like MTT assay and the MTS assay, they are colorimetric assays for measuring the activity of enzymes that reduce MTT or close dyes (XTT, MTS, WSTs) to formazan dyes, giving a purple yellow. A main application allows assessing the viability (cell counting) and the proliferation of cells (cell culture assays). It can also be used to determine cytotoxicity of potential medicinal agents and toxic materials, since those agents would stimulate or inhibit cell viability and growth.
3. This kit is very convenience to use. It is unnecessary to use isotope, to wash and collect cells, as well as to solute formazan. The entire step can be done in only one 96-well plate.
4. No interrupt with Phenol red and serum.
5. WST-1 is not toxic to cells. After adds WST-1, it can be read by coated wells repeatedly in different time. In this way, the detect time will be more flexible to find the best detect time.
6. The kit components are sufficient for performing up to 100 assays.