WRN Antibody - middle region (Phospho-T802) (OAAB08322)

Catalog No: OAAB08322

Size:400ul

WRN Antibody (Center T802) (Cat. #OAAB08322) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the WRN antibody detected the WRN protein (arrow).

WRN Antibody (Center T802) (Cat. #OAAB08322) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, FC
• Additional Information: Precautions: WRN Antibody (Center T802) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair Alleviates stalling of DNA polymerases at the site of DNA lesions Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity)
  Cellular Location: Nucleus, nucleolus. Nucleus.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This WRN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 787-816 amino acids from the Central region of human WRN.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  WRN is strongly supported by BioGPS gene expression data to be expressed in K562

• Application Info: WB: 1:1000
  FC: 1:10~50
  IHC-P: 1:100

Target Info

• Gene Symbol: WRN
• Alias Symbols: RECQ3, RECQL2, RECQL3
• NCBI Gene Id: 7486
• Protein Name: Werner syndrome ATP-dependent helicase
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q14191
• Protein Accession #: NP_000544.2
• Molecular Weight: 162461 Da