Catalog No: OKWB00167
Size:96T
Price: $450.00
SKU
OKWB00167
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Datasheets/ManualsPrintable datasheet for WRB ELISA Kit (Human) (OKWB00167)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)87-100%85-103%85-96%86-95%
EDTA Plasma (n=5)86-101%87-100%87-94%88-97%
Heparin Plasma (n=5)85-98%83-96%86-96%85-100%
ELISA Kit PrincipleAviva Systems Biology WRB ELISA Kit (Human) (OKWB00167) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WRB has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WRB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample WRB captured in the well.
ELISA Kit Range18.75-1200pg/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)85-10497
EDTA Plasma (n=5)86-10593
Heparin Plasma (n=5)88-10497
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
WRB Microplate96 Wells (12 x 8 Well strips)
WRB Lyophilized Standard2 x 1.2 ng
100X WRB Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity11.25 pg/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolGET1
Gene Full Nameguided entry of tail-anchored proteins factor 1
Alias SymbolsCHD5;congenital heart disease 5 protein;guided entry of tail-anchored proteins factor 1;tail-anchored protein insertion receptor WRB;tryptophan rich basic protein;Tryptophan-rich basic protein;WRB.
NCBI Gene Id7485
Protein NameTail-anchored protein insertion receptor WRB
Description of TargetReceptor for ASNA1/TRC40-mediated insertion of tail-anchored (TA) proteins into the ER membrane.
Uniprot IDO00258
Protein Accession #NP_001139690
Nucleotide Accession #NM_001146218
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