Catalog No: OKWB00167
Size:96T
Price: $450.00
SKU
OKWB00167
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for WRB ELISA Kit (Human) (OKWB00167) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology WRB ELISA Kit (Human) (OKWB00167) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WRB has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WRB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample WRB captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 18.75-1200pg/ml | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Mean Intra-Assay Precision <10% Mean Inter-Assay Precision <12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 11.25 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Type: Quantitative Sandwich ELISA |
Gene Symbol | GET1 |
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Gene Full Name | guided entry of tail-anchored proteins factor 1 |
Alias Symbols | CHD5;congenital heart disease 5 protein;guided entry of tail-anchored proteins factor 1;tail-anchored protein insertion receptor WRB;tryptophan rich basic protein;Tryptophan-rich basic protein;WRB. |
NCBI Gene Id | 7485 |
Protein Name | Tail-anchored protein insertion receptor WRB |
Description of Target | Receptor for ASNA1/TRC40-mediated insertion of tail-anchored (TA) proteins into the ER membrane. |
Uniprot ID | O00258 |
Protein Accession # | NP_001139690 |
Nucleotide Accession # | NM_001146218 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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