immunofluorescence

  1. Product Protocols: TFDP1 Antibody (P100820_P050) tested with HCT116 by Immunofluorescence

    TFDP1 Antibody (P100820_P050) tested with HCT116 by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) -Block with 5% goat serum, 1% BSA, and 0.2% fish gelatin -Incubate at 4 °C overnight Primary Addition...
  2. Product Protocols: BRCC3 Antibody (ARP61990_P050) tested with MCF7 by Immunofluorescence

    BRCC3 Antibody (ARP61990_P050) tested with MCF7 by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) -Block with 5% goat serum, 1% BSA, and 0.2% fish gelatin -Incubate at 4 °C overnight Primary Addition...
  3. Product Protocols: CDYL Antibody (ARP49096_P050) tested with MD MB231 by Immunofluorescence

    CDYL Antibody (ARP49096_P050) tested with MD MB231 by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) -Block with 5% goat serum, 1% BSA, and 0.2% fish gelatin -Incubate at 4 °C overnight Primary Addition...
  4. Product Protocols: CDYL Antibody (ARP49096_P050) tested with HeLa by Immunofluorescence

    CDYL Antibody (ARP49096_P050) tested with HeLa by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) -Block with 5% goat serum, 1% BSA, and 0.2% fish gelatin -Incubate at 4 °C overnight Primary Addition...
  5. Product Protocols: TRIM33 Antibody (ARP39214_P050) tested with MCF7 by Immunofluorescence

    TRIM33 Antibody (ARP39214_P050) tested with MCF7 by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) -Block with 5% goat serum, 1% BSA, and 0.2% fish gelatin -Incubate at 4 °C overnight Primary Addition...
  6. Product Protocols: BRD4 Antibody (ARP39076_P050) tested with Hela by Immunofluorescence

    BRD4 Antibody (ARP39076_P050) tested with Hela by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) -Block with 5% goat serum, 1% BSA, and 0.2% fish gelatin -Incubate at 4 °C overnight Primary Addition -Remove...
  7. Product Protocols: HOXB7 Antibody (ARP34393_T100) tested with SKOV3 by Immunofluorescence

    HOXB7 Antibody (ARP34393_T100) tested with SKOV3 by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) -Block with 5% goat serum, 1% BSA, and 0.2% fish gelatin -Incubate at 4 °C overnight Primary Addition -Remove block solution...
  8. Product Protocols: MSX2 Antibody (ARP31398_P050) tested with MCF7 by Immunofluorescence

    MSX2 Antibody (ARP31398_P050) tested with MCF7 by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) -Block with 5% goat serum, 1% BSA, and 0.2% fish gelatin -Incubate at 4 °C overnight Primary Addition -Remove block...
  9. Product Protocols: CSTF2T Antibody (ARP40809_P050) tested with SKOV3 by Immunofluorescence

    CSTF2T Antibody (ARP40809_P050) tested with SKOV3 by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) -Block with 5% goat serum, 1% BSA, and 0.2% fish gelatin -Incubate at 4 °C overnight Primary Addition -Remove block...
  10. TEAD4 Antibody (ARP33426_P050) tested with HeLa by Immunofluorescence

    TEAD4 antibody - middle region (ARP33426_P050) Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) -Block with 5% goat serum, 1% BSA, and 0.2% fish gelatin -Incubate at 4 °C overnight Primary Addition -Remove block...

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