Hela Cells

  1. Product Review: SENP3 antibody - N-terminal region (ARP55322_P050) in HeLa cells using Immunohistochemistry

    Product Page Link: SENP3 antibody - N-terminal region (ARP55322_P050) Data provided by Dr. Michael Matunis of Johns Hopkins University Protocol: 1. Wash HeLa cells 1X with PBS 2. Add fix for 30 min RT (2% Formaldehyde in PBS) 3. Wash 2X with PBS 4. Put in -20C Acetone for 3 minutes to permeabilize 5. Wash 2X with PBS 6. Put in...
  2. Product Review: NUP35 antibody - N-terminal region (ARP52578_P050) in HeLa cells using Immunohistochemistry

    Product Page Link:  NUP35 antibody - N-terminal region (ARP52578_P050) Data provided by Dr. Michael Matunis of John Hopkins University Protocol: 1. Wash HeLa cells 1X with PBS 2. Add fix/perm solution (20 mM PIPES pH=6.8, 4% Formaldehyde, 0.2% TNX100, 10 mM EGTA, 1 mM MgCl2) and let incubate for 10 min. at RT 3. Wash 2X with PBS 4. Put in...
  3. Product Review: NUP50 antibody - C-terminal region (ARP52288_P050) in HeLa cells using Immunohistochemistry

    Product Page Link:  NUP50 antibody - C-terminal region (ARP52288_P050) Data provided by Dr. Michael Matunis of John Hopkins University Protocol: 1. Wash HeLa cells 1X with PBS 2. Add fix/perm solution (20 mM PIPES pH=6.8, 4% Formaldehyde, 0.2% TNX100, 10 mM EGTA, 1 mM MgCl2) and let incubate for 10 min. at RT 3. Wash 2X with PBS 4. Put in...
  4. Product Review: NUP62 antibody - N-terminal region (ARP46179_P050) in HeLa cells using Immunohistochemistry

    Product Page Link: NUP62 antibody - N-terminal region (ARP46179_P050) Data provided by Dr. Michael Matunis of John Hopkins University Protocol: 1. Wash HeLa cells 1X with PBS 2. Add fix/perm solution (20 mM PIPES pH=6.8, 4% Formaldehyde, 0.2% TNX100, 10 mM EGTA, 1 mM MgCl2) and let incubate for 10 min. at RT 3. Wash 2X with PBS 4. Put...
  5. Product Review: NUP98 antibody - N-terminal region (ARP39251_P050) in HeLa cells using Immunohistochemistry

    Product Page Link: NUP98 antibody - N-terminal region (ARP39251_P050) Data provided by Dr. Michael Matunis of John Hopkins University Protocol: 1. Wash HeLa cells 1X with PBS 2. Add fix/perm solution (20 mM PIPES pH=6.8, 4% Formaldehyde, 0.2% TNX100, 10 mM EGTA, 1 mM MgCl2) and let incubate for 10 min. at RT 3. Wash 2X with PBS 4. Put...
  6. Product Review: GBA antibody - C-terminal region (ARP61322_P050) in rat organs, fibroblasts and HeLa cells using Western Blot.

    Product Page Link: GBA antibody - C-terminal region (ARP61322_P050) Data provided by Dr. Andrea Balreira Protocol: SDS-PAGE and WB Cell culture samples (fibroblasts and HeLa cells) were lysed and rat organs were homogenized, and proteins were quantified by the Bradford technique. 100ug of each sample were subjected to SDS-PAGE and electroblotted onto a nitrocellulose membrane. Membranes were blocked with %5...
  7. Product Review: MCM8 antibody – N-terminal region (ARP36658_P050)using Western Blot

    Product Page Link: MCM8 antibody - N-terminal region (ARP36658_P050) Data Provided by Dr. Marcel Mechali Protocol: - primary antibody over nigth at 4°C at 1:2000 in PBS-Tween 0,1%-BSA 2,5% - 3x10 minutes of wash with PBS-Tween 0,1% - secondary antibody from GE healthcare (ref NA934V) at 1:3000 in PBS-Tween 0,1%, 1h at room   température - 3x10 minutes of wash with...
  8. Product Review: WRNIP1 antibody – N-terminal region (ARP36470_P050) using Western Blot

    Product Page Link: WRNIP1 antibody - N-terminal region (ARP36470_P050) Data Provided by Dr. Marcel Mechali Protocol: - primary antibody over nigth at 4°C at 1:2000 in PBS-Tween 0,1%-BSA 2,5% - 3x10 minutes of wash with PBS-Tween 0,1% - secondary antibody from GE healthcare (ref NA934V) at 1:3000 in PBS-Tween 0,1%, 1h at room   température - 3x10 minutes of wash with...
  9. Product Review: PMS2 antibody – middle region (ARP56117_P050) in HeLa cells using Western Blot.

    Product Page Link: PMS2 antibody - middle region (ARP56117_P050) Data Provided by Dr. Marcel Mechali Protocol: - primary antibody over nigth at 4°C at 1:2000 in PBS-Tween 0,1%-BSA 2,5% - 3x10 minutes of wash with PBS-Tween 0,1% - secondary antibody from GE healthcare (ref NA934V) at 1:3000 in PBS-Tween 0,1%, 1h at room   température - 3x10 minutes of wash with...
  10. Product Review: CDH1 antibody - middle region (ARP59265_P050) using HeLa whole cell lysate in Western Blots

    Product Page Link: CDH1 antibody - middle region (ARP59265_P050) Data provided by: Dr. Chang, MIT Summary: Cdh1 antibody - specific band at correct molecular weight when used at 1:200 for 1 hour on WB of whole cell lysate. What type of experimental sample are you using and how did you prepare it? Cell extracts from control HeLa and HeLas transfected with...

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