HeLa

Please go here to see the protocol used for Aviva's TFPI Antibody (OAEB02071) Product datasheet can be found here. WB information: Approx 50kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 35.0kDa according to NP_006278.1). In transfected HEK293 transiently expressing TFPI bands of approx. 38kDa to 45kDa are observed. These bands are not observed in...

Please go here to see the protocol used for Aviva's TFPI Antibody (OAEB02071) Product datasheet can be found here. WB information: Approx 50kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 35.0kDa according to NP_006278.1). In transfected HEK293 transiently expressing TFPI bands of approx. 38kDa to 45kDa are observed. These bands are not observed in...

Please go here to see the protocol used for Aviva's DAP3 Antibody (OAEB01341) Product datasheet can be found here. WB information: Approx 40kDa band observed in lysates of cell line HeLa (calculated MW of 45.6kDa according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293...

Please go here to see the protocol used for Aviva's DAP3 Antibody (OAEB01341) Product datasheet can be found here. WB information: Approx 40kDa band observed in lysates of cell line HeLa (calculated MW of 45.6kDa according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293...

CDYL Antibody (ARP49096_P050) tested with HeLa by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) -Block with 5% goat serum, 1% BSA, and 0.2% fish gelatin -Incubate at 4 °C overnight Primary Addition...

BRD4 Antibody (ARP39076_P050) tested with Hela by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) -Block with 5% goat serum, 1% BSA, and 0.2% fish gelatin -Incubate at 4 °C overnight Primary Addition -Remove...

TEAD4 antibody - middle region (ARP33426_P050) Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) -Block with 5% goat serum, 1% BSA, and 0.2% fish gelatin -Incubate at 4 °C overnight Primary Addition -Remove block...

SF3A1 antibody - N-terminal region (ARP40615_P050) Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) -Block with 5% goat serum, 1% BSA, and 0.2% fish gelatin -Incubate at 4 °C overnight Primary Addition -Remove block...

PPP1R10 antibody - N-terminal region (ARP40413_P050) Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) -Block with 5% goat serum, 1% BSA, and 0.2% fish gelatin -Incubate at 4 °C overnight Primary Addition -Remove block...

GNL3L antibody - N-terminal region (ARP58799_P050) Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) -Block with 5% goat serum, 1% BSA, and 0.2% fish gelatin -Incubate at 4 °C overnight Primary Addition -Remove block...