Product Review: NPM1 antibody – C-terminal region (ARP34114_T100) for human optic nerve head for immunohistochemistry

Product Review: NPM1 antibody – C-terminal region (ARP34114_T100) for human optic nerve head for immunohistochemistry
NPM1 antibody – C-terminal region (ARP34114_T100) for human optic nerve head
NPM1 antibody – C-terminal region (ARP34114_T100) for immunohistochemistry

Nucleophosmin expression in the epithelium and stroma of the human cornea.

Nucleophosmin (cryo) = 1/100; Alexa fluor 546 Goat anti-rabbit. Lumican = 1/1000

Frozen sections:
Rehydrate with PBS
Acetone extraction for 3 min. @ -20oC
2 x 5mins washes in 1xPBS
Blocking @ 37oC for 30min
Primary Ab for 1-2hrs @37oC or overnight at 4oC
2x 5mins washes in 1xPBS
Secondary Ab for 30min @ 37oC
3x 5mins washes in 1xPBS
5min @ RT Counterstain w/ DAPI
2x 5mins washes in 1xPBS

We used a 5min antigen retrieval method in the Pelco Biowave (Ted Pella) using the IHCTek epitope retrieval solution.

I would rate this antibody 4/5

Data submitted by: Geraint Parfitt, UC Irvine.  Immunostaining by Geraint Parfitt, Korey Reid and Yilu Xie

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