Product Page Link: MCM8 antibody - N-terminal region (ARP36658_P050)

Data Provided by Dr. Marcel Mechali

Protocol:

- primary antibody over nigth at 4°C at 1:2000 in PBS-Tween 0,1%-BSA 2,5%
- 3x10 minutes of wash with PBS-Tween 0,1%
- secondary antibody from GE healthcare (ref NA934V) at 1:3000 in PBS-Tween 0,1%, 1h at room   température
- 3x10 minutes of wash with PBS-Tween 0,1%

-20 ug of extract per lane.

- ECL revelation