Primary Antibody Dilution: 2ug/ml
Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution: 1: 20,000
Image Submitted by: Yuzhi Chen
University of Arkansas for Medical Science
Additional protocol details: 2N HCL 30 min, washed in 3x PBS, stained for 1 hour and 30 minutes, with 1 ug/50 ul antibody and incubated in Alexa goat anti-rabbit 594 for 1 hour. Images taken with a 40x objective.