Technical Questions

  1. What is the sequence of the peptide used to raise this antibody?

    Aviva has made over 40,000 rabbit polyclonal antibodies using short peptide sequences, usually around 14aa in length.  It is Aviva's policy not to disclose the sequence of the immunizing peptide.  However, we do offer two indicators of the location of the antibody binding epitope; 1) Antibody title.  Each protein sequence is split into three parts; N-terminal, middle region or C-terminal. ...
  2. Does Aviva Systems Biology have an antibody to [species] [gene name]?

    Aviva has made over 40,000 rabbit polyclonal antibodies using short peptide sequences, usually around 14aa in length.  Only about half of these antibodies are searchable on our website.  For a complete listing of all the antibodies (validated and unvalidated) that may be reactive to your protein of interest, we have created an antibody BLAST search tool to search by protein...
  3. Technical Question: What is the origin and make-up of the cut off control reagent?

    Product Page for Rubella IgM Capture ELISA Kit (96 Wells) As I understand it, the wells of the plate are coated with anti-hIgM mAb.  Only a portion (if any) of the bound IgM in a given sample will be anti-Rubella IgM.  The minimum amount of specific anti-Rubella IgM that can be detected with this kit is determined by this cut...
  4. Technical Question: Has the COX2 antibody been used successfully with feline COX2?

    List of COX2 antibodies Unfortunately, none of our COX-2 antibodies have been used with feline samples yet.  I think the best way to find an antibody that is specific for feline COX-2 would be to use our new antibody BLAST tool.  This will find homology between your sequence and the peptides used to generate all of our >30,000 antibodies.  Results...
  5. Technical Question: Could you please provide reconstitution and storage instructions?

    Please see our Reconstitution & Storage Instructions webpage for exactly this kind of information: //www.avivasysbio.com/protocols-procedures/reconstitution-storage-instructions
  6. Technical Question: What is the concentration of your control human plasma?

    Product Page for Human Prealbumin (Transthyretin) ELISA Kit (96 wells) The range for human plasma may be 0.08 – 0.3 mg/ml
  7. Technical Question: Does this CHAC1 antibody work in mouse?

    Product Page for CHAC1 antibody - C-terminal region (ARP42623_P050) Unfortunately, this antibody has not been tested with mouse samples.  It does show 100% homology with the mouse CHAC1 sequence (http://www.uniprot.org/uniprot/Q8R3J5) so it’s expected to react.
  8. Technical Question: What cell types were used to validate the ANO6 antibody in mouse? What was the Western Blot Procedure?

    Product Page for Ano6 antibody - middle region (ARP44759_P050) From the results of our lab, this antibody was tested on mouse and rat brain, heart, kidney, liver, lung, intestine and muscle.  The tissues that showed the correct sized band came from mouse and rat liver. Also, our western blotting protocol can be found here: //www.avivasysbio.com/protocols-procedures/wb#WBIB
  9. Technical Question: Why is there a difference between the predicted and the actual molecular weight for CHRNA1?

    Product Page for CHRNA1 antibody - C-terminal region (AVARP13084_P050) There seems to be a difference between the predicted and actual MW of this protein. My concern is with the molecular weight of the observed band. Several blots I have seen online indicate an observed molecular weight band at ~51 or ~55kD by western blot (using other antibodies against CHRNA1). Could the loss...
  10. Technical Question: What is the cross reactivity of SHH to DHH and IHH?

    Product Page for SHH antibody - N-terminal region (ARP44235_P050) Based on the sequence of the 14aa immunizing peptide used to generate our anti-SHH antibody (ARP44235_P050), here are the homologies to the protein sequences you have sent: Desert Hedgehog DHH:http://www.ncbi.nlm.nih.gov/protein/O43323.1 57% homology Indian Hedgehog IHH: http://www.ncbi.nlm.nih.gov/protein/Q14623.4 77% homology

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