Category Archives: Immunoflourescence Protocols

Immunoflourescence Protocols

Product Protocols: TFDP1 Antibody (P100820_P050) tested with HCT116 by Immunofluorescence

Posted on December 30, 2013 by salaghehband

TFDP1 Antibody (P100820_P050) tested with HCT116 by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer … Continue reading →

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Product Protocols: BRCC3 Antibody (ARP61990_P050) tested with MCF7 by Immunofluorescence

Posted on December 30, 2013 by salaghehband

BRCC3 Antibody (ARP61990_P050) tested with MCF7 by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer … Continue reading →

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Product Protocols: CDYL Antibody (ARP49096_P050) tested with MD MB231 by Immunofluorescence

Posted on December 30, 2013 by salaghehband

CDYL Antibody (ARP49096_P050) tested with MD MB231 by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer … Continue reading →

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Product Protocols: CDYL Antibody (ARP49096_P050) tested with HeLa by Immunofluorescence

Posted on December 30, 2013 by salaghehband

CDYL Antibody (ARP49096_P050) tested with HeLa by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer … Continue reading →

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Product Protocols: TRIM33 Antibody (ARP39214_P050) tested with MCF7 by Immunofluorescence

Posted on December 30, 2013 by salaghehband

TRIM33 Antibody (ARP39214_P050) tested with MCF7 by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer … Continue reading →

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Product Protocols: BRD4 Antibody (ARP39076_P050) tested with Hela by Immunofluorescence

Posted on December 30, 2013 by salaghehband

BRD4 Antibody (ARP39076_P050) tested with Hela by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% … Continue reading →

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Product Protocols: HOXB7 Antibody (ARP34393_T100) tested with SKOV3 by Immunofluorescence

Posted on December 30, 2013 by salaghehband

HOXB7 Antibody (ARP34393_T100) tested with SKOV3 by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) -Block … Continue reading →

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Product Protocols: MSX2 Antibody (ARP31398_P050) tested with MCF7 by Immunofluorescence

Posted on December 30, 2013 by salaghehband

MSX2 Antibody (ARP31398_P050) tested with MCF7 by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) … Continue reading →

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Product Protocols: CSTF2T Antibody (ARP40809_P050) tested with SKOV3 by Immunofluorescence

Posted on December 20, 2013 by salaghehband

CSTF2T Antibody (ARP40809_P050) tested with SKOV3 by Immunofluorescence Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) … Continue reading →

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TEAD4 Antibody (ARP33426_P050) tested with HeLa by Immunofluorescence

Posted on November 6, 2013 by salaghehband

TEAD4 antibody – middle region (ARP33426_P050) Cell Seeding -Allow cells to attach for 24 hours at 37 °C and 5% CO2 Fix, Wash, Block -Fix cells using 4% PFA for 30 mins -Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100) … Continue reading →

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