Antibody Pairs

  1. Product Protocol: TG antibody used to evaluate tumor cytotoxicity and endothelial rac inhibition induced by tnp-470 in anaplastic thyroid cancer (OASA03319)

    Specificity: THYROGLOBULINProduct Page: TG antibody (OASA03319)Antibody Pair Available: OASA03318 Capture OASA03319 DetectionExperiment Type: ImmunohistochemistryProtocol: Immunohistochemistry was done for cleaved caspase-3 (apoptosis), Ki-67 (proliferation), thyroglobulin (thyroid cell marker), and CD31 (vascular marker). The protocol for immunohistochemistry is as follows: 5-μm-thick paraffin-sectioned slides (Histobond; Statlab, Lewisville, TX) were first heated for 10 min at 55°C. Slides were then deparaffinized with xylene, rehydrated...
  2. Product Protocol: TNFSF10 antibody used to evaluate type i interferon negatively controls plasmacytoid dendritic cell numbers in vivo (OASA07564)

    Specificity: CD253Product Page: TNFSF10 antibody (OASA07564)Antibody Pair Available: OASA07564 Capture OASA07567 DetectionExperiment Type: Mice and treatments, Antibodies, flow cytometry, and cell sortingProtocol: 1. Mice and treatments: All animal studies were approved by the Washington University Animal Studies Committee. IFNAR−/− mice (C57BL/6 background) were provided by A. French (Washington University, St. Louis, MO). Bim−/− and Bid−/− mice (Bouillet et al., 1999...
  3. Product Protocol: IL5 antibody used to evaluate ultrastructural immunogold localization of interleukin 5 to the crystalloid core compartment of eosinophil secondary granules in patients with atopic asthma (OASA07901)

    Specificity: IL-5Product Page: IL5 antibody (OASA07901)Antibody Pair Available: OASA07901 Capture OASA07903 DetectionExperiment Type: lmmunogold Labeling to Demonstrate IL-5Protocol: Grids were rinsed for 10 min in 0.14% glycine in 0.1 M PBS, pH 7.2. The following sequence of steps was used to stain the grids, by placing them into reagent drops: (a) 10% normal goat serum (NGS) in PBS, 30 min...
  4. Product Protocol: RABBIT ANTI HUMAN TIMP-1 antibody used to evaluate urinary matrix metalloproteinases reflect renal damage in anti-neutrophil cytoplasm autoantibody-associated vasculitis (OASA11651)

    Specificity: TIMP-1Product Page: RABBIT ANTI HUMAN TIMP-1 antibody (OASA11651)Antibody Pair Available: OASA11651 Capture OASA11652 DetectionExperiment Type: ELISAProtocol: Urinary and plasma levels of MMP-2, MMP-9, and TIMP-1 were determined by ELISA. In brief, for MMP-9 and TIMP-1 ELISA, 96-well plates (M129A, Greiner) were precoated with F(ab)2 fragments of goat anti-mouse IgG-Fc (Jackson, West Grove, PA) in 0.1 M carbonated buffer (pH...
  5. Product Protocol: RABBIT ANTI HUMAN TIMP-1:Biotin antibody used to evaluate urinary matrix metalloproteinases reflect renal damage in anti-neutrophil cytoplasm autoantibody-associated vasculitis (OASA11652)

    Specificity: TIMP-1Product Page: RABBIT ANTI HUMAN TIMP-1:Biotin antibody (OASA11652)Antibody Pair Available: OASA11651 Capture OASA11652 DetectionExperiment Type: ELISAProtocol: Urinary and plasma levels of MMP-2, MMP-9, and TIMP-1 were determined by ELISA. In brief, for MMP-9 and TIMP-1 ELISA, 96-well plates (M129A, Greiner) were precoated with F(ab)2 fragments of goat anti-mouse IgG-Fc (Jackson, West Grove, PA) in 0.1 M carbonated buffer (pH...
  6. Product Protocol: BMP2 antibody used to evaluate vitreous modulation of gene expression in low-passage human retinal pigment epithelial cells (OASA07470)

    Specificity: BMP-2Product Page: BMP2 antibody (OASA07470)Antibody Pair Available: OASA07470 Capture OASA07472 DetectionExperiment Type: Immunohistochemistry, Immunoblot AnalysisProtocol: 1. Immunohistochemistry: RPE cells were grown for 48 hours in control medium or medium containing 25% vitreous. To inhibit secretion of extracellular proteins, Brefeldin A (Sigma-Aldrich, St. Louis, MO) was then added to the medium to a final concentration of 5 ng/mL, and the...
  7. Product Protocol: MOUSE ANTI CANINE HEARTWORM antibody used to evaluate survey of canine dirofilaria immitis infection in new caledonia (OASA00429)

    Specificity: CANINE HEARTWORMProduct Page: MOUSE ANTI CANINE HEARTWORM antibody (OASA00429)Antibody Pair Available: OASA00429 Capture OASA00431 DetectionExperiment Type: Laboratory Diagnostics (enzyme-linked immunoabsorbent assay)Protocol: To evaluate the prevalence of heartworm infection in dogs, two antigen test kits were used in a blind study using serum drawn from 59 stray dogs from the area. The efficiency of chemoprophylaxis was assessed on 15 military...
  8. Product Protocol: CXCL9 antibody used to evaluate synergistic induction of cxcl9 and cxcl11 by toll-like receptor ligands and interferon-gamma in fibroblasts correlates with elevated levels of cxcr3 ligands in septic arthritis synovial fluids (OASA07659)

    Specificity: CXCL9Product Page: CXCL9 antibody (OASA07659)Antibody Pair Available: OASA07659 Capture OASA07660 DetectionExperiment Type: ImmunoassaysProtocol: Sandwich enzyme-linked immunosorbent assays (ELISAs) for human CXCL9 and CXCL11 were developed in our laboratory. Microtiter plates (Maxisorp, Nunc-Immuno Plate, Invitrogen) were coated with 1.67 μg/ml monoclonal mouse anti-human CXCL9 antibody (Ab; R&D Systems) or with 670 ng/ml polyclonal rabbit anti-human CXCL11 Ab (PeproTech) in phosphate-buffered...
  9. Product Protocol: IL10 antibody used to evaluate t regulatory type 1 cells in squamous cell carcinoma of the head and neck: mechanisms of suppression and expansion in advanced disease (OASA07859)

    Specificity: IL-10Product Page: IL10 antibody (OASA07859)Antibody Pair Available: OASA07859 Capture OASA07860 DetectionExperiment Type: Immunofluorescence of tumor tissuesProtocol: Tissue samples were embedded in OCT, and 5 μm frozen sections were cut in a cryostat, dried, fixed for 10 min in cold acetone and ethanol (1:1), and again dried in air. The following monoclonal anti-human antibodies were used for staining: CD4/FITC (diluted...
  10. Product Protocol: TNFSF10 antibody used to evaluate targeting multiple pathways in gliomas with stem cell and viral delivered s-trail and temozolomide (OASA07567)

    Specificity: CD253Product Page: TNFSF10 antibody (OASA07567)Antibody Pair Available: OASA07564 Capture OASA07567 DetectionExperiment Type: S-TRAIL ELISAProtocol: To determine the concentration of S-TRAIL released by AAV-S-TRAIL transduced cells, 0.4 × 106 TRAIL-resistant 293T cells were transduced with increasing MOI of control virus (Gluc-DsRed2) or S-TRAIL. Twenty-four hours later, medium was collected and TRAIL protein concentration in the medium of transduced cells was...

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