WNT7B ELISA Kit (Mouse) (OKCA02442)

Catalog No: OKCA02442 (Formerly GWB-J1CB7V)

Size:96 Wells

Mouse WNT7B ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	93	85-105
  1:2	89	84-96
  1:4	95	90-100
  1:8	95	87-101

• ELISA Kit Principle: Aviva Systems Biology Wnt7b ELISA Kit (Mouse) (OKCA02442) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Wnt7b has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Wnt7b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Wnt7b captured in the well.
• ELISA Kit Range: 0.312 - 20 ng/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	97	93-101
  EDTA Plasma (n=4)	94	89-99

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • Wnt7b Microplate: 96 Wells (12 x 8 Well strips)
  • Wnt7b Lyophilized Standard: 2
  • 100X Biotinylated Wnt7b Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.078 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: WNT7B
• Alias Symbols: Wnt-7, Wnt-7b
• NCBI Gene Id: 22422
• Protein Name: Protein Wnt-7b
• Description of Target: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
• Uniprot ID: P28047
• Protein Accession #: NP_001157105
• Nucleotide Accession #: NM_001163633