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Catalog No: OKEH08611
Size:96T
Price: $875.00
SKU
OKEH08611
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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WNT7B ELISA Kit (Chicken) (OKEH08611)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityChicken, Gallus gallus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology WNT7B ELISA Kit (Chicken) (OKEH08611) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WNT7B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WNT7B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample WNT7B captured in well.
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 106% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=7.3% (n = 20)
Mean Inter-assay CV%: <=12.0% (n = 20)
ELISA Kit Component
ComponentAmount
WNT7B Microplate96 Wells (12 x 8 Well strips)
WNT7B Lyophilized Standard2
100X Biotinylated WNT7B Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
::Subcellular Location: Secreted Extracellular space Extracellular matrix
::Unigene: Gga.32583
SMR: Q3L254
String: 9031.ENSGALP00000029875
::KEGG: gga:427937
Pfam: PF00110
Reconstitution and StorageStore as indicated in product manual.
SpecificityNatural and recombinant Chicken Protein Wnt-7b
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolWNT7B
Gene Full NameWnt family member 7B
Alias Symbolsprotein Wnt-7b;wingless-type MMTV integration site family, member 7B.
NCBI Gene Id427937
Protein NameQ3L254
Description of TargetProtein Wnt-7b
Uniprot IDhttps://www.uniprot.org/uniprot/Q3L254
Protein Accession #NP_001032351.1
Nucleotide Accession #NM_001037274.1
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