WNT5A ELISA Kit (Mouse) (OKEH04420)

Catalog No: OKEH04420

Size:96T

Mouse WNT5A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Wnt5a ELISA Kit (Mouse) (OKEH04420) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Wnt5a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Wnt5a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Wnt5a captured in the well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 5.5% (n = 20)
  Mean Inter-assay CV%: <= 8.1% (n = 20)
• ELISA Kit Component:
  • Anti-Wnt5a Microplate: 96 Wells (12 x 8 Well Strips)
  • Wnt5a Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Wnt5a Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.156 ng/mL
• Specificity: Natural and recombinant Mouse Protein Wnt-5a
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for WNT5A ELISA Kit (Mouse) (OKEH04420) on CiteAb

Target Info

• Gene Symbol: Wnt5a
• Gene Full Name: wingless-type MMTV integration site family, member 5A
• Alias Symbols: 8030457G12Rik;protein Wnt-5a;wingless-related MMTV integration site 5A;Wnt-;Wnt-5a.
• NCBI Gene Id: 22418
• Protein Name: P22725
• Description of Target: Protein Wnt-5a
• Uniprot ID: https://www.uniprot.org/uniprot/P22725
• Protein Accession #: NP_001243153.1
• Nucleotide Accession #: NM_001256224.1
• Protein Size (# AA): 380
• Molecular Weight: 42 KDa