WNT5A Antibody (OABB01528)

Catalog No: OABB01528

Size:100UG

Anti-Wnt5a Picoband antibody
All lanes: Anti-WNT5A(OABB01528) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 41 kDa
Observed bind size: 41 kDa

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Bovine|Dog|Human|Monkey|Mouse|Rabbit|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: WB: The detection limit for Wnt5a is approximately 0.25ng/lane under reducing conditions.
  Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Protein Wnt-5a is a protein that in humans is encoded by the WNT5A gene. This gene is a member of the WNT gene family. The WNTs comprise a large class of secreted proteins that control essential developmental processes such as embryonic patterning, cell growth, migration, and differentiation. It is mapped to 3p14.3. WNT5A is highly expressed in the dermal papilla of depilated skin. This protein plays an essential role in regulating developmental pathways during embryogenesis. And this protein may also play a role in oncogenesis. WNT5A and DVL can promote axon differentiation mediated by the PAR3-PAR6-aPKC complex.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: E.coli-derived human Wnt5a recombinant protein (Position: D184-D303). Human Wnt5a shares 100% amino acid (aa) sequence identity with both mouse and rat Wnt5a.
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat: Mouse
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: Mouse, Rat: By Heat
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Protein Wnt-5a(WNT5A) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Target Info

• Gene Symbol: WNT5A
• Gene Full Name: Wnt family member 5A
• Alias Symbols: epididymis secretory sperm binding protein;hWNT5A;protein Wnt-5a;wingless-type MMTV integration site family, member 5A;WNT-5A protein.
• NCBI Gene Id: 7474
• Protein Name: Protein Wnt-5a
• Description of Target: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (By similarity). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed:15735754). Mediates motility of melanoma cells (PubMed:17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity).
• Uniprot ID: P41221
• Molecular Weight: 42339 MW