WNT5A Antibody (OAAD00302)

Catalog No: OAAD00302

Size:0.1ml

western blot analysis using WNT5A mouse mAb against HEK293 (1) and WNT5A-hIgGFc transfected HEK293 cell lysate (2).

Immunohistochemical analysis of paraffin-embedded human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) showing cytoplasmic and extracellular matrix localization using WNT5A mouse mAb with DAB staining.

Confocal immunofluorescence analysis of PC-12 cells using WNT5A mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.

Product Info

• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: 6F2
• Isotype: IgG1
• Host: Mouse
• Application: IF, IHC, WB
• Reconstitution and Storage: Store at 4C, for long term storage, store at -20C.
• Immunogen: Purified recombinant fragment of human XBP1 expressed in E. Coli.
• Formulation: Ascitic fluid containing 0.03% sodium azide.
• Reference: 1. J Biol Chem. 2009 May 29;284(22):14904-13.
  2. Neoplasia. 2009 May;11(5):436-47.
  3. Clin Cancer Res. 2009 Jun 1;15(11):3834-41.

Target Info

• Alias Symbols: hWNT5A
• NCBI Gene Id: 7474
• Description of Target: This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
• Uniprot ID: P41221
• Molecular Weight: 42.3kDa