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Catalog No: OKEH01547
Size:96T
Price: $600.00
SKU
OKEH01547
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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WNT3A ELISA Kit (Human) : 96 Wells (OKEH01547)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH01547
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology WNT3A ELISA Kit (Human) (OKEH01547) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for WNT3A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WNT3A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample WNT3A captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 91%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.4% (n = 20)
Mean Inter-assay CV%: < 7.7% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-WNT3A Microplate96 Wells (12 x 8 Well strips)
WNT3A Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated WNT3A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.067 ng/mL
SpecificityNatural and recombinant Human Protein Wnt-3a
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolWNT3A
Gene Full NameWnt family member 3A
Alias Symbolsprotein Wnt-3a;wingless-type MMTV integration site family, member 3A.
NCBI Gene Id89780
Protein NameP56704
Description of TargetProtein Wnt-3a
Uniprot IDhttps://www.uniprot.org/uniprot/P56704
Protein Accession #NP_149122.1
Nucleotide Accession #NM_033131.3
Protein Size (# AA)352
Molecular Weight39 kDa
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