WNT16 ELISA Kit (Mouse) (OKCA02187)

Catalog No: OKCA02187

Size:96 Wells

Mouse WNT16 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	103	101-105
  Serum (n=4)	1:2	96	93-99
  Serum (n=4)	1:4	89	86-92
  Serum (n=4)	1:8	98	94-102

• ELISA Kit Principle: Aviva Systems Biology Wnt16 ELISA Kit (Mouse) (OKCA02187) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WNT16 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WNT16 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample WNT16 captured in well.
• ELISA Kit Range: 62.5 pg/mL-4,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	106	101-111
  EDTA plasma (n=4)	92	89-96

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-WNT16 Microplate: 96 Wells (12 x 8 Well strips)
  • WNT16 Lyophilized Standard: 2 x 4 ng
  • 100X Biotinylated WNT16 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: WNT16
• Alias Symbols: E130309I19Rik
• NCBI Gene Id: 93735
• Protein Name: Protein Wnt-16
• Description of Target: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
• Uniprot ID: Q9QYS1
• Protein Accession #: NP_444346
• Nucleotide Accession #: NM_053116