WNT1 ELISA Kit (Human) (OKEH04852)

Catalog No: OKEH04852 (Formerly GWB-XYM11S)

Size:96T

Human WNT1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology WNT1 ELISA Kit (Human) (OKEH04852) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WNT1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WNT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample WNT1 captured in well.
• ELISA Kit Range: 12.5-800pg/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • WNT1 Microplate: 96 Wells (12 x 8 Well Strips)
  • WNT1 Standard: 2
  • Sample Diluent: 1 x 20 mL
  • WNT1 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 9.8 pg/mL
• Specificity: Natural and recombinant Human Proto-oncogene Wnt-1
• Publications:

  PSPC1-interchanged interactions with PTK6 and β-catenin synergize oncogenic subcellular translocations and tumor progression. Nat Commun. 10, 5716 (2019). 31844057

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for WNT1 ELISA Kit (Human) (OKEH04852) on CiteAb

Target Info

• Gene Symbol: WNT1
• Gene Full Name: Wnt family member 1
• Alias Symbols: BMND16;INT1;OI15;proto-oncogene Int-1 homolog;proto-oncogene Wnt-1;wingless-type MMTV integration site family member 1;wingless-type MMTV integration site family, member 1 (oncogene INT1).
• NCBI Gene Id: 7471
• Protein Name: P04628
• Description of Target: Proto-oncogene Wnt-1
• Uniprot ID: https://www.uniprot.org/uniprot/P04628
• Protein Accession #: NP_005421.1
• Nucleotide Accession #: NM_005430.3
• Protein Size (# AA): 371
• Molecular Weight: 41 kDa