WNK1 Antibody (Phospho-Thr60) (OASG07578)

Catalog No: OASG07578

Size:100 uL

Western Blot using 293

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC-P, ELISA
• Additional Information: Tissue Specificity: Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
  Cellular Location: Cytoplasm.
• Reconstitution and Storage: Store at -20°C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human WNK1 around the phosphorylation site of T60. Location: 40-120aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Phospho-Thr60
• Specificity: Phospho-WNK1 (T60) Polyclonal Antibody detects endogenous levels of WNK1 protein only when phosphorylated at T60.
• Application Info: WB: 1:500~2000
  IHC: 1:100~300
  ELISA: 1:5000

Target Info

• Gene Symbol: WNK1
• Gene Full Name: WNK lysine deficient protein kinase 1
• Alias Symbols: KDP, PSK, p65, HSN2, HSAN2, PRKWNK1, PPP1R167
• NCBI Gene Id: 65125; 232341; 116477
• Description of Target: This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.
• Uniprot ID: Q9H4A3, P83741, Q9JIH7
• Molecular Weight: 230 kDa